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Figure 4 | Molecular Cancer

Figure 4

From: Epigenetic inactivation of miR-9 family microRNAs in chronic lymphocytic leukemia - implications on constitutive activation of NFκB pathway

Figure 4

Effect of 5-Aza-2′-deoxycytidine (5-AzadC) treatment on I83-E95 and WAC3CD5+ cells. U-/M-MSP analysis of miR-9-3 promoter methylation status and stem-loop RT-qPCR analysis of the pri-miR-9-3 expression in untreated (A) I83-E95, (B) WAC3CD5+ cells and after treatment with 0.5 μM 5-Azad C for 5 days. ΔCt, Ct pri-miR-9-3-Ct GAPDH. GAPDH was used as reference for data analysis of pri-miR-9-3 expression by 2-ΔΔCT method. 5-AzadC treatment led to the progressive demethylation of miR-9-3 promoter, and re-expression of the pri-miR-9-3 in I83-E95 and WAC3CD5+ cells. Columns represent mean ± standard deviation of triplicate qPCRs.

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Molecular Cancer

ISSN: 1476-4598