Immunoblot of ADAMTS-1 in tumor (T) and adjacent normal tissue (N). Four bands were identified as ADAMTS-1 (A, arrows). Ductal carcinomas and matched adjacent tissues were obtained from twenty-eight patient donors and analyzed. The 80 kDa band/tubulin ratio was significantly lower in tumors as compared to normal tissues (B). No differences were found among other bands obtained from neoplastic and normal tissues (not illustrated). The boxed area shows the 80 kDa bands that were evaluated by densitometry. The asterisks indicate significant data (p < 0.05).