ADAMTS-1 was more abundant in normal tissue stroma as compared to tumor stroma. ADAMTS-1 staining is shown for normal tissue, invasive ductal carcinoma of grades IIA, IIB, IIIA and IIIC and metastatic lymph nodes (A). When the total area fraction was analyzed by immunohistochemistry, ADAMTS-1 showed no statistically significant difference in staining between normal and tumor tissues (B). However, a decreasing trend was observed in high-grade types (IIIA and IIB). Subgroup analysis revealed a significant decrease in ADAMTS-1 expression in triple negative tumors as compared to normal tissues and ER- or PR-positive tumors (C). The labeling intensity profile (D) shows that the expression of ADAMTS-1 in normal tissue was prominent in both epithelial cells and stroma. In contrast, ADAMTS-1 staining was discrete or absent in tumor stroma (D, boxed area), and this discrete or absent staining pattern was more evident in higher grades of malignancy (D, boxed area). Scale bar: 50 μm.