ADAMTS-1 expression in cell lysates and conditioned medium from MCF7 and MDA-MB-231 cells. These cells showed reduced expression of ADAMTS-1 following siRNA treatment. The lysates and conditioned media were analyzed by immunoblot, and an 80-kDa band was prevalent in the conditioned medium from both cells lines, but more prominently in MDA-MB-231. A 40-kDa smaller band was predominant in the cell lysates. The immunoblot also illustrated the efficiency of ADAMTS-1 siRNA knockdown. These experiments were carried out at least six times, and similar results were consistently obtained.