Figure 5

ADAMTS-1 knockdown increased the velocity of MDA-MB-231 cells, as shown by time-lapse video microscopy. The cells were transfected with either ADAMTS-1 shRNA-GFP (treated) or non-silencing shRNA-GFP (controls). The fluorescent images were superimposed with the MDA-MB-231 cell trajectories (A, color tracks) and are illustrated as manual markings on the same cell at different time points. The cells were recorded after 4.5 hours. The treated cells (B) demonstrated longer trajectories over time as compared to the controls (C). The velocity of the treated cells was also higher as compared to the controls (D). The asterisks indicate significant data in comparison to the controls (p < 0.001). The results in B represent the mean ± standard error obtained from 6 experiments. Scale bar: 100 μm. ADAMTS-1 knockdown decreased the velocity of MCF7 cells, as shown by time-lapse video microscopy. The cells were transfected with either ADAMTS-1 shRNA-GFP (treated) or non-silencing shRNA-GFP (controls). The fluorescent images were then superimposed with MCF7 cell trajectories (color tracks) and are illustrated as manual markings on the same cell at different time points (E). The cells were recorded after 4.5 hours. The treated cells (F) presented shorter trajectories over time as compared to the controls (G), and the velocity of the treated cells was lower in comparison to the controls (H). The asterisks indicate significant data in comparison to the controls (p < 0.01). The results in D represent the mean ± standard error obtained from 6 experiments. Scale bar: 100 μm.