Figure 7

Increased cell migration and invasion was stimulated by decreased expression of ADAMTS-1 and was partially rescued by a VEGF functional blocking antibody (A, B). ADAMTS-1 knockdown increased the migration and invasion of MDA-MB-231 cells. The migration of MDA-MB-231 cells with ADAMTS-1 silenced by siRNA increased two-fold as compared to the control. The addition of an anti-VEGF blocking antibody partially rescued this migration to control levels (A). Invasion assays were carried out in Boyden chambers coated with Matrigel (B). Cells with silenced ADAMTS-1 demonstrated three-fold increased levels of invasion as compared to the controls. Invasion was partially rescued to control levels in cells pretreated with the VEGF blocking antibody. The asterisks indicate significant data (*p < 0.05, **p < 0.01, ***p < 0.001). The results represent the mean ± standard error of three experiments carried out at least three times.