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Figure 2 | Molecular Cancer

Figure 2

From: Timp1 interacts with beta-1 integrin and CD63 along melanoma genesis and confers anoikis resistance by activating PI3-K signaling pathway independently of Akt phosphorylation

Figure 2

A tight Timp1/CD63/β1-integrin complex is formed only in tumorigenic cell lines. A. β1-integrin expression pattern was analyzed in murine melan-a, 4C, 4C11- and 4C11+ cell lines by Western blotting using a specific polyclonal antibody. Note the shift of β1-integrin eletrophoretic migration in the metastatic 4C11+ melanoma cell line. The same membrane was reprobed with anti-actin antibody as an endogenous control. B. CD63 protein expression was evaluated by flow cytometry using antibody specific. C. Membrane-enriched protein extracts from melan-a, 4C, 4C11-, and 4C11+ cell lines were immunoprecipitated with anti-CD63 and anti-β1-integrin and analyzed by Western blotting with anti-Timp1 and anti-β1-integrin. ma: non-tumorigenic melanocytes melan-a; 4C: pre-malignant melanocytes; 4C11-: non-metastatic melanoma cells; 4C11+: metastatic melanoma cells. *p < 0.05, **p < 0.01.

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Molecular Cancer

ISSN: 1476-4598