Ectopic expression of miR-497 decreases cell viability and increases apoptotic rates in vitro. (A) Neuroblastoma MYCN-amplified cell lines Kelly (n=3), CHP-212 (n=3) and non-MYCN-amplified SK-N-AS (n=3) were transfected with miR-497 mimics/scrambled negative control (Neg Control) oligonucleotides. Viability of cells was measured by MTS assay at 24 hr, 48 hr, 72 hr and 96 hr post transfection. (B) Mean percentage of annexin V+ Kelly, CHP-212 and SK-N-AS cells, from at least three independent experiments at 96 hr post transfection with miR-497 mimic/Scrambled NC. (C) Caspase 3/7 activation was measured at 72 h post transfection (n=3, each × 5 technical replicates) in Kelly, CHP-212 and SK-N-AS cell lines, (D) Representative Annexin V Scatter plots for neuroblastoma MYCN-amplified Kelly and CHP-212 cells following transfection with miR-497 mimics/scrambled negative control (Neg Control) oligonucleotides. (E) Images of miR-497 over-expressing cells vs scrambled negative controls (Neg Control) in Kelly, CHP-212 and SK-N-AS.