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Figure 3 | Molecular Cancer

Figure 3

From: MicroRNA-497 increases apoptosis in MYCN amplified neuroblastoma cells by targeting the key cell cycle regulator WEE1

Figure 3

MiR-497 targets the 3′UTR of WEE1 in neuroblastoma cells. (A) Two conserved 7-mer seed matches with hsa-miR-497 in WEE1 3′ UTR. (B) WEE1 mRNA expression levels 24 hours after transfection with miR-497 compared to negative controls in neuroblastoma MYCN-amplified cell lines Kelly (n=3), CHP-212 (n=3) and non-MYCN-amplified SK-N-AS (n=3). (C) WEE1 protein levels, following transfection with miR-497 mimics or scrambled negative control, determined by western blot of protein extraction at 96 hrs. (D) Densitometry analysis of WEE1 protein levels following miR-497 over-expression in Kelly, CHP-212 and SK-N-AS cells. (E) Kelly cells were co-transfected with miR-497 mimics or Scrambled negative control and either wild-type (WT) or mutated (mut) WEE1 3′UTR reporter constructs. Luciferase activity was determined 48 hrs post transfection.

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Molecular Cancer

ISSN: 1476-4598