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Figure 5 | Molecular Cancer

Figure 5

From: MicroRNA-497 increases apoptosis in MYCN amplified neuroblastoma cells by targeting the key cell cycle regulator WEE1

Figure 5

Cells transfected with miR-497 mimics or siWEE1 followed by CDDP treatment. (A) Neuroblastoma MYCN-amplified cell lines Kelly, CHP-212 and non-MNA SK-N-AS were transfected with miR-497 mimics/scrambled negative control (Neg Control) oligonucleotides. At 48 hr post transfection, cells were treated for 24 hr with 5 μg/ml concentration of CDDP. Media was replaced for a further 24 hrs. Mean percentage of annexin V+ Kelly, CHP-212 and SK-N-AS cells, from at least three independent experiments at 96 hr post transfection (B) Neuroblastoma MYCN-amplified cell lines Kelly, CHP-212 and non-MNA SK-N-AS were transfected with siWEE1 /siNegative control (siNeg Control). At 48 hrs post transfection, cells were treated for 24 hr with 5 μg/ml concentration of CDDP. Media was replaced for a further 24 hr.Mean percentage of annexin V+ Kelly, CHP-212 and SK-N-AS cells, from at least three independent experiments at 96 hr post transfection (C) Representative Annexin V Scatter plots for neuroblastoma MYCN-amplified Kelly cells following transfection with miR-497 mimics/scrambled negative control (Neg Control) oligonucleotides and 5 μg/ml CDDP treatment. (D) Representative Annexin V Scatter plots for neuroblastoma MYCN-amplified Kelly cells following transfection with siWEE1 /siNegative control (siNeg Control) and 5 μg/ml CDDP treatment.

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