CRIP1 knockdown increases the invasive potential of T47D and BT474 breast cancer cells in vitro . (A) A quantification of the invasion assay of nontransfected T47D cells or BT474 cells (mock) compared with transiently transfected T47D cells or BT474 cells 48 h post-transfection (T47D cells) and 72 h post-transfection (BT474 cells). The mean values and standard deviations of two independent experiments are shown. (B) Western blot analysis showing the expression of cleaved (84 kDa band) MMP9 in supernatants of nontransfected T47D cells (mock) and T47D cells with transiently downregulated CRIP1 expression using effective siRNAs and siRNAGAPDH (positive control). The mean values of three independent experiments are shown.