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Figure 3 | Molecular Cancer

Figure 3

From: MicroRNA-32 (miR-32) regulates phosphatase and tensin homologue (PTEN) expression and promotes growth, migration, and invasion in colorectal carcinoma cells

Figure 3

Alteration of miR-32 expression changes PTEN protein expression. (A) MiR-32 expression was detected by qRT-PCR. SW480 cells transfected with miR-32 mimics showed an increase in miR-32 expression, while HCT-116 cells transfected with miR-32 inhibitor resulted in significantly decreased miR-32 expression. *P < 0.05 when compared with corresponding negative control. (B) Representative pictures of PTEN protein expression detected by western blot. (C) Quantitative analysis showed that PTEN protein expression in SW480 cells transfected with miR-32 mimics decreased when compared with miR-32 mimics-NC or blank control. PTEN protein increased in miR-32 inhibitor transfected HCT-116 cells when compared with miR-32 inhibitor-NC or blank control. *P < 0.05 (D) Transfected cells had no alternation in PTEN mRNA level. Data were the means of three measurements and the bars represented SD of the mean.

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Molecular Cancer

ISSN: 1476-4598