Transwell invasion assay. SW480 and HCT-116 cells were transfected with miR-32 mimics and miR-32 inhibitor, respectively. (A, B) CRC cells penetrating the membrane were fixed and 0.1% crystal violet stained after 24 h as described in experimental procedures. (C) CRC cells penetrating the membrane were represented as the fold over blank control. *P < 0.05, as compared miR-32 mimics with its other two groups, or miR-32 inhibitor with other two groups. Data were the means of three measurements and the bars represented SD of the mean.