Concordance between SnoN and PLSCR1 RNA expression in ovarian cancer cell lines. (A) Oncomine analysis of the Ovarian TCGA data set shows increased copy number of PLSCR1 (left panel) and SnoN (right panel) in ovarian serous cystadenocarcinomas (582 specimens) in contrast to normal ovary (127 specimens). (B) Correlation analysis between PLSCR1 and SnoN copy number in ovarian cancer specimens. (C) RNA was harvested from normal (T80 and T29) and malignant (OVCAR8, OVCA429, HEY, and SKOV3) ovarian cell lines. PLSCR1 mRNA levels were quantified by quantitative PCR. The results are displayed as relative RNA-fold change. Results are representative of duplicate experiments. (D) Correlation analyses between PLSCR1 and SnoN RNA and DNA copy number in ovarian cancer cell lines. (E) Western blotting was performed using antibodies against the proteins indicated using cell lysates isolated from the cell lines described in (C). Results are representative of duplicate experiments. (F) Correlation analyses between PLSCR1 and SnoN protein expression.