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Figure 2 | Molecular Cancer

Figure 2

From: Phospholipid Scramblase 1, an interferon-regulated gene located at 3q23, is regulated by SnoN/SkiL in ovarian cancer cells

Figure 2

Knockdown of SnoN Reduces PLSCR1 mRNA Levels. (A) RNA was isolated from HEY cells 48-hours post-transfection with SnoN siRNA. PLSCR1 (left panel) and SnoN (right panel) mRNA levels were quantified by real-time PCR. Results are representative of duplicate experiments. (B) T80 cells were transfected with the PLSCR1 promoter followed by either SnoN knockdown (B) or 24 h TGFβ treatment (C). Cell lysates were harvested and luciferase activity was quantified. Results are representative of duplicate experiments. (D) RNA was isolated from T80 cells treated with TGFβ (3 and 18 h). PLSCR1 (left panel) and SnoN (right panel) mRNA levels were quantified by real-time PCR. Results are representative of duplicate experiments. (E) T80 cells were mock transfected or transiently transfected with either wild type PLSCR1 (WT-PLSCR1, 0.25 μg to 2.0 μg) or C/A PLSCR1 mutant (C/A-PLSCR1, 0.25 μg to 2.0 μg). Cell lysates were analyzed by western blotting for the proteins indicated. Results are representative of duplicate experiments.

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