Figure 3

SnoN modulates IFN-2α-mediated induction of PLSCR1 mRNA. (A) HEY cells were treated with 3000 IU/ml IFN-2α (15 min – 24 h). Cell lysates were harvested followed by western blotting for the indicated proteins. Results are representative of triplicate experiments. (B) HEY cells were treated with 3000 IU/ml IFN-2α (0, 1, and 3 hours). RNA was isolated followed by real-time PCR analysis to quantify PLSCR1 mRNA levels. Results are representative of duplicate experiments. HEY cells were treated with 3000 IU/ml IFN-2α (18 h) followed by immunofluorescence staining (C) or subcellular fractionation of cytoplasmic and nuclear fractions (D) which were analyzed by western blotting for the proteins indicated. Results are representative of duplicate experiments. (E) HEY cells were transfected with non-targeting or SnoN siRNA followed by IFN-2α treatment (3 h). Forty-eight hours post-transfection, total RNA was isolated followed by quantitation of PLSCR1 (E) and SnoN (F) mRNA by real-time PCR. Results are representative of duplicate experiments.