Ad-eIF5A1 and Ad-eIF5A1
infection results in accumulation of unhypusinated eIF5A1. A) Two-dimensional gel electrophoresis of lysate from A549 cells forty-eight hours after infection with either Ad-LacZ, Ad-eIF5A1, or Ad-eIF5AK50A followed by western blotting with eIF5A1 antibody. Cells were treated with the DHS inhibitor, GC7, as a positive control. A short exposure of blots from eIF5A1-overexpresing lysates is included for comparison. B) The relative abundance of the different post-translationally modified forms of eIF5A1 and eIF5A1K50A, including spot a (acetylated K47/K50 or acetylated K47/A50), spot b (K47/K50 or K47/A50), and spot c (K47/Hypusine 50), was determined by densitometry.