Ad-eIF5A1 infection upregulates Bax and Bid. A549 lung carcinoma cells were infected with adenovirus expressing eIF5A1 at increasing multiplicities of infection (MOI). Adenoviral infection with Ad-eIF5A1 (5A) was at MOI’s of 5, 10, 25 or 80 while Ad-LacZ (L) was given at an MOI of 80. Forty-eight hours later, the cell lysate was harvested and used for western blot analysis of Bcl-2 family proteins. The data is representative of three independent experiments.