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Figure 7 | Molecular Cancer

Figure 7

From: Role of p38 and JNK MAPK signaling pathways and tumor suppressor p53 on induction of apoptosis in response to Ad-eIF5A1 in A549 lung cancer cells

Figure 7

A549 lung carcinoma cells are more susceptible to eIF5A1-induced apoptosis than normal lung cells. A549 lung carcinoma cells or WI38 normal lung fibroblasts cells were infected at an MOI of 80 with adenovirus expressing LacZ, eIF5A1, or eIF5A1K50A. Four hours after infection, the media was replaced with fresh media and cells were harvested forty-eight and seventy-two hours later hours later. (A) A549 and WI38 cells infected with adenovirus were labeled with Annexin/PI and the percentage of cells undergoing apoptosis was determined by flow cytometry analysis. The data shown is the mean of 3 independent experiments. Statistical significance in comparison to paired A549 cells is indicated (* p < 0.05; ** p < 0.01; *** p < 0.001). (B) Forty-eight hours after infection, cell lysates were harvested and the expression of eIF5A, MAPK/SAPK proteins, and Bcl-2 was examined by western blot analysis. The blots shown are representative of three independent experiments. Quantification of expression of phosphorylated p38 (C) and phosphorylated p42/p44 ERK MAPK (D) relative to expression of unphosphorylated total protein.

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