Figure 4
From: Molecular changes induced by the curcumin analogue D6 in human melanoma cells
Western blot analysis. Validation by western blotting of selected gene products expression. LB24 melanoma cells were grown to semi-confluence, treated (+) or not (−) for 24 hours with 10 μM D6, then harvested and lysed as described in Methods. Cell lysates were analysed by western blotting for the expression levels of selected proteins modulated by D6 treatment: (A) p53 targets: p21, GADD45A and Noxa; (B) cell cycle regulators: cyclin B, cdc25, cyclin F; (C) growth signals stimulating proteins: PI3K, p-Akt, c-kit; (D) proapoptotic proteins: DDIT3, Bcl10. Protein levels were quantified by Image J Software and normalized over (ratio) the GAPDH protein levels used as internal control. Data are reported as percentage of protein expression in D6 treated cells compared to untreated ones (100%).