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Figure 2 | Molecular Cancer

Figure 2

From: 6-Formylindolo (3,2-b)carbazole (FICZ) enhances retinoic acid (RA)-induced differentiation of HL-60 myeloblastic leukemia cells

Figure 2

FICZ upregulates: AhR, AhR target genes and MAPK cascade components in the presence of RA. Cell lysates collected 48 h after initiation of the experiments were resolved on 12% polyacrylamide gels. 25 μg protein was loaded per well. In RA-treated cells, FICZ upregulates the amount of Cyp1A2, and p47phox, AhR protein expression and pERK1/2, pMEK1/2 and pS621 RAF phospho-protein in the presence of RA compared to cells treated with RA alone. Like pS621 RAF, terminal domain phosphorylated RAF is also upregulated. Total ERK1/2, MEK1/2, RAF are unchanged. The loading control was GAPDH.

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