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Figure 5 | Molecular Cancer

Figure 5

From: 6-Formylindolo (3,2-b)carbazole (FICZ) enhances retinoic acid (RA)-induced differentiation of HL-60 myeloblastic leukemia cells

Figure 5

FICZ and other AhR ligands modulate MAPK signaling cascade regulatory molecules. Cell lysates collected after 48 h of RA treatment were resolved on 12% polyacrylamide gels. 25 μg protein was loaded per well. Similarly to FICZ, β-NF upregulates the amount of Fgr, Lyn, VAV1 protein and p-Y416 pan-SFK, pY507 Lyn, and pY458 p85PI3K phospho-protein, whereas the antagonist does not upregulate those markers Comparing lanes 4 and 8 FICZ and β-NF, the two AhR agonists, have apparently similar signaling profiles in RA-treated cells, which is not shared by α-NF, an AhR antagonist, lane 6). The agonists by themselves without RA do not have this effect. GAPDH is the loading control.

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