Expression and sub-cellular localization of an epigenetic regulator, co-activator arginine methyltransferase 1 (CARM1), is associated with specific breast cancer subtypes and ethnicity

Table 2 Summary of statistical tests for each of the 11 variables investigated

	Simple linear regression tests for cyt-CARM1				Simple linear regression tests for nuc-CARM1
	N	P-value	Beta	SE(Beta)	N	P-value	Beta	SE(Beta)
CK5/6*	574	*0.0188*	0.099	0.042	573	0.7506	0.0178	0.0559
EGFR*	574	*0.0061*	0.1377	0.0501	573	0.7031	0.0254	0.0667
Tumor grade*	416	*0.0102*	0.128	0.0496	415	0.1030	0.1059	0.0648
	Cumulative logistic regression tests for cyt-CARM-1				Cumulative logistic regression tests for nuc-CARM-1
	N	P-value	OR	95% OR CI	N	P-value	OR	95% OR CI
ER	482	0.3053	0.834	0.588 - 1.181	481	0.6592	1.079	0.769 - 1.515
PR	480	0.8028	0.954	0.656 - 1.385	479	0.9608	1.009	0.702 - 1.452
HER2**	477	0.0023	1.948	1.269 - 2.989	476	<0.0001	2.596	1.702 - 3.959
Age	443	0.0669	0.989	0.977 - 1.001	629	0.5913	0.997	0.985 - 1.009
Tumor size	427	0.252	1.039	0.973 - 1.110	596	0.5936	1.018	0.955 - 1.085
	One-way ANOVA tests for cyt-CARM-1				One1-way ANOVA tests for nuc-CARM-1
	N	P-value	RMSE	Significant differences	N	P-value	RMSE	Significant differences
Molecular subtype**	475	0.0004	0.6657	(HER2, Basal) > LumA	474	0.0004	0.872	HER2 > (LumA, Basal)
Histological subtype	576	0.0319	0.69	(none found)	575	0.1049	0.9146	(none found)
Ethnicity**	534	0.003	0.7019	Afr > (Cauc, AfrAm)	533	0.0001	0.0001	(Cauc, AfrAm) > Afr.

Depending on the structure of the data for each variable, one of three statistical tests were used; Simple Linear Regression, Cumulative Logistic Regression or ANOVA. See methods for details of stats applications. Asterisks and bold font P-values indicate significant associations at 0.05 level. All significant associations are described in detail in the text. RMSE root mean squared error; CI confidence interval; SE standard error; OR odds ratio; E(Y) expected values of CARM1 (Y) at indicated level of variable’s score (0–3).

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