Figure 2
From: Thymoquinone attenuates tumor growth in ApcMin mice by interference with Wnt-signaling
TQ induces tumor-specific apoptosis in APCMinmice. TQ-low and TQ-high significantly increased the number of apoptotic cells (by TUNEL assay) within SI polyps but not in the normal mucosa (A). Bar graphs display the mean number (± SD) of apoptotic cells per field of view (FoV, n=12). *p<0.05, **p<0.01; by ANOVA (2-sided Dunnett); paired T-test was used to compare the normal mucosa to neoplastic cells within each group (only significant for TQ-high). Representative images of normal mucosa (upper panel) and polyp tissue (lower panel) in untreated (B), TQ-high (C, F), and piroxicam treated APCMin mice (D). DNase I treated positive control (E). DAPI (blue) and incorporated fluorescein-12-dUTP (green) signals were imaged at 400x using a confocal fluorescence microscope (LSM 5 Exciter; analysis software: LSM image examiner, Zeiss, Jena, Germany).