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Figure 1 | Molecular Cancer

Figure 1

From: Activation of Akt pathway by transcription-independent mechanisms of retinoic acid promotes survival and invasion in lung cancer cells

Figure 1

ATRA activates the Akt pathway through non-genomic mechanisms in A549 cells .(A) Left, A549 cells were serum-starved for 18 h, treated or non-treated (NT) with 5 μM of ATRA, for the times indicated. Right, A549 cells were preincubated for 1 h with 3 μM of BMS493 before ATRA treatment and total extracts were prepared. The phosphorylated form of Akt and total proteins were detected by western blot using specific antibodies. The bottom graph represents the densitometric values of Akt phosphorylation of three independent experiments (means ± SEM, *P < 0.05; **P < 0.001 compared with non-treated cells (NT) (analysis of variance and Newman-Keuls test). (B) A549 cells were serum-starved and treated with ATRA with or without BMS493 for 48 h. Total proteins were detected by western blot.

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Molecular Cancer

ISSN: 1476-4598