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Figure 2 | Molecular Cancer

Figure 2

From: Activation of Akt pathway by transcription-independent mechanisms of retinoic acid promotes survival and invasion in lung cancer cells

Figure 2

ATRA promotes Akt activation mediated by RARα-Akt interaction. (A) RARα was immunoprecipitated from A549 cells treated or non-treated with 5 μM of ATRA for 15 min. Immunoprecipitated RARα and associated protein were detected by western blot. Control refers to immunoprecipitation performed with an Erk1 antibody. The bottom graph shows the results of densitometric analyses of Akt bound to RARα obtained from three independent experiments (means ± SEM, *P < 0.05 compared with non-treated cells (NT) assessed by t test analysis). (B) RARα was immunoprecipitated from A549 cells transfected with EGFP-APPL1 or empty vector and treated with 5 μM of ATRA for 15 min. Association of RARα with Akt was detected by western blot using specific antibodies. Image shows one representative experiment of three independent.

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Molecular Cancer

ISSN: 1476-4598