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Figure 6 | Molecular Cancer

Figure 6

From: Activation of Akt pathway by transcription-independent mechanisms of retinoic acid promotes survival and invasion in lung cancer cells

Figure 6

Inactive form of Akt (K179M) blocks the ATRA-dependent survival effect. (A) A549 cells were transfected with Myr-Akt, Akt-K179M or empty vector and subsequently treated or non-treated with 5 μM of ATRA for 48 h. Subsequently, DNA fragmentation was detected by TUNEL according to the manufacturer’s instructions. Control cells were non-treated. The apoptotic cells are stained brown. (B) Percentages of TUNEL-positive cells were quantified by counting 100 cells from three random microscopic fields. Means ± SEM, *P < 0.05; **P < 0.001 compared with non-treated cells (NT) (analysis of variance and Newman-Keuls test). Bar, 20 μm.

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Molecular Cancer

ISSN: 1476-4598