The interaction of celecoxib with MDR transporters enhances the activity of mitomycin C in a bladder cancer cell line

Table 2 Biological evaluation of MDR inhibitors and reference compounds

EC₅₀± SEM,^a(μM)
Compounds	Caco-2 [³H] vinblastine transport inhibition	MDCK-MDR1 Calcein-AM	MDCK-MRP1 Calcein-AM	MDCK-BCRP Calcein-AM
Celecoxib	30 ± 2.0	46.9 ± 2.5	10.1 ± 0.5	24.1 ± 1.2
Mitomycin C	10 ± 0.5	N.D.^b	N.D.^b	N.D.^b
MK-571 ^c			2.85 ± 0.25
Verapamil ^c	20^d			3.65 ± 0.2

^aData ± SEM are the mean of three independent determinations (samples in triplicate).
^bMitomycin C activity was not determined because in the experimental conditions quenched the spectroscopic properties of the probe.
^cReference compounds for MDR pumps.
^dSee Colabufo et al. [40].

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