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Figure 1 | Molecular Cancer

Figure 1

From: POU1F1 is a novel fusion partner of NUP98 in acute myeloid leukemia with t(3;11)(p11;p15)

Figure 1

Cytogenetic and fluorescence in situ hybridization studies of the AML patient. The bone marrow sample was cultured for 24 h following standard procedures. Chromosome banding analysis of the bone marrow was performed after Leichmann’s staining and the karyotype was described according to ISCN [4]. A) Bone marrow karyotype obtained by GTL banding technique exhibiting a t(3;11)(p11;p15) as the sole chromosome abnormality, suggesting the involvement of the NUP98 gene located at 11p15. Derivative chromosomes 3 and 11 are marked with arrows. BAC clones for NUP98 and POU1F1 were selected using the UCSC Human Genome Browser and obtained from the BACPAC Resource Center. B) Dual color, dual fusion FISH analysis performed on metaphases using BAC probes for NUP98 (CTD-3082 N2, RP11-161I4) labeled in green and for POU1F1 (CTD-2372C6) labeled in red, showed the presence of fusion signals on der(3) and der(11) and isolated red and green signals on normal chromosomes 3 and 11, respectively.

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