The interaction between hMSH4 and eIF3f. (A) Yeast-two hybrid analysis of the hMSH4-eIF3f interaction. A series of hMSH4 truncation mutants were utilized to determine the eIF3f-interacting domain on hMSH4. Positive interactions were ascertained by the transcription activation of ADE2 and HIS3 reporter genes. (B) Co-IP analysis of hMSH4-eIF3f interaction in human cells. Immunoblotting with α-tubulin was used as a loading control. (C) Co-IP analysis of the interaction between eIF3f and hMSH4sv. Full-length hMSH4 and hMSH4sv were expressed separately with eIF3f and the respective interactions were analyzed by co-IP. (D) GST pull-down analysis of hMSH4-eIF3f interaction and determination of the hMSH4-interacting domain on eIF3f. The full-length eIF3f and four truncated fragments were expressed as GST-fusion proteins. GST pull-down experiments were performed with Glutathione-Sepharose 4B beads.