Figure 6From: MutS homologue hMSH4: interaction with eIF3f and a role in NHEJ-mediated DSB repairEffects of eIF3f-hMSH4 on IR-induced cell cycle arrest. (A) Cell cycle analysis of 293T, 293T/eIF3f, and 293T/eIF3f-hMSH4 cells treated with different doses of IR. Cell cycle analysis was conducted either at 12Â hrs or 24Â hrs post-IR treatment, and percentages of cells in the G2/M phase are indicated. (B) Effect of IR treatment on eIF3f-hMSH4 interaction. 293T/eIF3f cells were transfected to express Myc-hMSH4, and cells were then irradiated with 10Â Gy IR at 48Â hrs post-transfection. Cell lysates were prepared, 2Â hrs post-IR treatment, for subsequent co-IP analysis.Back to article page