Effects of eIF3f-hMSH4 on IR-induced cell cycle arrest. (A) Cell cycle analysis of 293T, 293T/eIF3f, and 293T/eIF3f-hMSH4 cells treated with different doses of IR. Cell cycle analysis was conducted either at 12 hrs or 24 hrs post-IR treatment, and percentages of cells in the G2/M phase are indicated. (B) Effect of IR treatment on eIF3f-hMSH4 interaction. 293T/eIF3f cells were transfected to express Myc-hMSH4, and cells were then irradiated with 10 Gy IR at 48 hrs post-transfection. Cell lysates were prepared, 2 hrs post-IR treatment, for subsequent co-IP analysis.