Figure 2

Effects of fludarabine on DU-145 cells. A, relative cell proliferation of DU-145 cells exposed for 48 h to increasing concentrations (0-10 μg/ml) of fludarabine. Cell cycle analysis (B), apoptosis (C) and plating efficiency (D) of DU-145 cells exposed for 48 h to 10 μg/ml of fludarabine. Dot plot of intracellular (Intra) to extracellular (Extra) levels of PCS- and S-miRNAs, expressed as fold changes in treated in comparison to untreated DU-145 cells with 10 μg/ml (E) or 1 μg/ml (F) of fludarabine. Upper left quarter: Intra down regulated, Extra up regulated; Upper right quarter: Intra up regulated, Extra up regulated; Lower left quarter: Intra down regulated, Extra down regulated; Lower right quarter: Intra up regulated, Extra down regulated. All data were shown as S.D. from three independent experiments (*P<0.05, **P<0.01, ***P<0.001, unpaired t-test).