Figure 3

PCS- and S-miRNAs levels in the exosomes isolated from DU-145 cells growth medium . A, fold change of PCS- (black bars) and S- (white bars) miRNAs levels in exosomes isolated from the growth medium of DU-145 fludarabine treated in comparison to untreated cells. B, relative miR-485-3p or miR-100 levels in exosomes of DU-145 cells transfected with miR-485-3p and miR-100 respectively versus those of miR-NC transfected cells. C, relative miR-485-3p or miR-100 levels in exosomes of DU-145 cells transfected with miR-485-3p or miR-100 respectively and then treated with fludarabine versus those of transfected but untreated cells. D, fold change of the expression levels of MDR1 and miR-485-3p in DU-145 cells at different time points after fludarabine treatment. E, cell proliferation of DU-145 cells transfected with antagomiRNA-485-3p (d-485-3p) or antagomiRNA-NC (d-NC) and after having been exposed (+flu) or unexposed (-flu) to fludarabine for 48 h. All data were shown as S.D. from three independent experiments (*P<0.05, **P<0.01, ***P<0.001, unpaired t-test).