Primer |       Sequence (5’-3’) | Product size (bp) |
---|---|---|
Primers for RT-PCR | ||
GAPDH | F:5'-CCACCCATGGCAAATTCCATGGCA-3' | 550 |
R:5'-TCTAGACGGCAGGTCAGGTCCACC-3' | ||
BCAT1 | F: 5'-CCAAAGCCCTGCTCTTTGTA-3' | 305 |
R: 5'-TGGAGGAGTTGCCAGTTCTT-3' | ||
c-Myc | F: 5'-CCTACCCTCTCAACGACAGC-3' | 179 |
R: 5'-TTCCTCCTCAGAGTCGCTGC-3' | ||
KRAS | F: 5'-GCAAAGACAAGACAGGGTG-3' | 264 |
R: 5'-GGTAAAAGCTAACAGTCTGC-3' | ||
MCAM | F: 5'- CTCCGCGTCTACAAAGCTCC-3' | 213 |
R: 5'- ACCACTCGACTCCACAGTCT-3' | ||
Primers for microsatellite loci of BCAT1 | ||
D12S1435 | F-CTTGTGCAACCCTCCCAC | 198 |
R-ATATGTGCTGTGAATACATCCACC | ||
D12S1617 | F-AGCCTGAGGGGCCACAT | 259 |
R-TGGGCAACTTGGATAAGAAACA | ||
RH44650 | F-AAGAATGTGTCTATTGCCAGCA | 146 |
R-CTCATGCCTCTGAAGGTTTTG |