Figure 2

Effect of TPL and ATF on the growth of tumour cells in vitro . (A) Left, A549 and HCT116 Cells were treated with TPL at different concentrations (0, 2.5, 5, 10, 20, 40 ng/mL) for 24 h, and the cell viability was assessed by MTT assay. Right, A549 and HCT116 cells were treated with ATF at different concentrations (0, 5, 10, 20, 40, 80 nM) for 24 h, and the cell viability was assessed by MTT assay. (B) Combined effects of TPL and ATF on growth of HCT116, MDA-MB-231, A549, HeLa, and HEK293 cells. HCT116, MDA-MB-231, A549, HeLa, and HEK293 cells were exposed to TPL (10 ng/mL) and/or ATF (10 nM) for 24 h. Combined treatment resulted in significant growth inhibition of tumour cells, more than that by either drug alone; the degree of cytotoxicity in normal cells was not significant. Each bar shows the mean ± SD of three independent experiments, performed in triplicate. *p < 0.05, **p < 0.01.