Skip to main content
Figure 3 | Molecular Cancer

Figure 3

From: Herbal compound triptolide synergistically enhanced antitumor activity of amino-terminal fragment of urokinase

Figure 3

Effects of TPL and ATF on tumour cell apoptosis and caspase activation. (A) A549 and HCT116 cells were exposed to TPL (10 ng/mL) and/or ATF (10 nM). Twenty-four hours later, all cells were harvested for flow cytometry analysis. Annexin V/PI-stained cells were analyzed with the percentages of apoptosis cells. The experiments were carried out independently in triplicate; representative data are shown. *p < 0.05, **p < 0.01. Annexin V/PI double staining profile of A549 cells is also included. (B) Cleaved caspase-3, PARP, cIAP, BAX, BAD, and BAK expressions in HCT116 cells under different treatment conditions. (C) Activity of caspase-3 and caspase-9 in HCT116 cells treated with TPL and ATF alone or in combination for 24 h. Data are presented as fold increases as determined by quantitative analysis. (D) Viability of HCT116 cells after treatment with caspase inhibitors. Cells were treated with inhibitors for 2 h before the 24 h treatments, after which cell viability was determined by MTT assay. Data are representative of three independent experiments. *p < 0.05, **p < 0.01.

Back to article page