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Figure 4 | Molecular Cancer

Figure 4

From: Herbal compound triptolide synergistically enhanced antitumor activity of amino-terminal fragment of urokinase

Figure 4

Effects of TPL and ATF on NF-κB and JNK signalling pathway. (A) HCT116 cells were treated with TPL and ATF alone or in combination for 24 h. Nuclear proteins were extracted and subjected to Western blotting for p65 detection. Lamin B was used as loading control and α-tubulin was used as a cytoplasmic protein marker to rule out cytoplasmic contamination in the nuclear fraction. (B) HCT116 cells were treated with TPL and ATF alone or in combination for 24 h. AKT, p-AKT, and c-FLIP proteins in whole cell lysates were determined with specific antibodies. GAPDH was used as loading control. (C) HCT116 cells were transfected with scramble siRNA or NF-κB p65 siRNA. 48 h later, cell extracts were applied for Western blotting analysis using NF-κB p65 and c-FLIP antibodies. (D) HCT116 cells were treated with TPL and ATF alone or in combination for 24 h. c-JUN, p-c-JUN and p-JNK protein in whole cell lysates were determined with specific antibodies.

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