Figure 5

Effect of TPL and ATF on cell cycle distribution. (A) HCT116 cells were treated with TPL (10 ng/mL) and/or ATF (10 nM). Twenty-four hours later, cells were fixed and stained with PI for flow cytometry as described in Materials and Methods. DNA histograms were modeled with CellQuest analysis software. The experiments were carried out in triplicate and representative data are shown. (B) Phase percentages for G0/G1, S, and G2/M are depicted by bar graph. Data represent mean values of triplicate samples.