Figure 7
The antitumor effect of TPL and ATF on the xenograft of colon cancer. HCT116 cells were injected subcutaneously into the dorsal flanks of athymic nude mice. When tumors reached a size of approximately 50 mm3, mice were i.p. with ATF and TPL or the combination every two day for a total of 21 day. (A) The tumor growth inhibitory effects of different treatments were compared. (B) At the end of the study, the excised tumors from each group were weighed. (C) Tumour double time of each group. (D) The weight of nude mice from each group did not change significantly during the experiment. (E) Determination of tumor necrosis after combined treatment with TPL and ATF. Tumour necrosis areas are shown by H&E staining and observed under light microscope (× 100). The viable tumor cells are indicated by a green arrow. (F) Quota of tumour necrosis. Tumour necrosis was determined by software Image J (NIH, USA). Two sections/mouse and three mice were prepared. (G, H) Blood vessel density within tumour was characterized by anti-CD31 immunostaining using anti-mouse CD31 monoclonal antibody (G) and determined by the average number of vessels in 3 regions of highest density at × 200 magnifications in each section (assay was repeated in 4 sections per mouse and 3 mice were tested) (H). All data are shown as mean ± SD. *p < 0.05, **p < 0.01.