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Figure 3 | Molecular Cancer

Figure 3

From: Flavokawain B, a kava chalcone, inhibits growth of human osteosarcoma cells through G2/M cell cycle arrest and apoptosis

Figure 3

FKB suppressed cell motility and invasiveness. A, Representative photomicrographs of scratch wounds were taken at 0 and 16 h after wound were made on 143B treated with FKB 7.5 μg/ml or control. Quantitative measurement of wound healed by ImageJ software showed a reduced cellular motility in FKB-treated 143B cells compared with control group. Columns, mean relative area (%) of wound healed; bars, SD. Experiments were replicated thrice. B, Cell invasion ability was analyzed by the Transwell chamber 36 h after FKB treatment at indicated concentration. The number of migrated cells was significantly decreased by FKB in a dose-dependent manner. C, Zymography and western blotting analysis of MMP-2 and MMP-9 from cultured medium of 143B cells treated with FKB. Both MMP-2 and MMP-9 activities were significant inhibited by FKB. Also the same inhibition effect of FKB on MMPs was observed on protein expression levels.

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