Pseudomonas aeruginosa NfsB and nitro-CBI-DEI – a promising enzyme/prodrug combination for gene directed enzyme prodrug therapy

Table 1 Nitroreductase candidates evaluated in this study

Protein name^a	Gene locus^b	% identity^c	Accession no.^d
NfsB_Pa	PA5190	25 (NfsB_Ec)	AAG08575.1
AzoR2_Pa	PA1962	41 (AzoR_Ec)	AAG05350.1
AzoR3_Pa	PA3223	45 (AzoR_Ec)	AAG06611.1
KefF_Pa	PA4975	37 (KefF_Ec)	AAG08360.1
MdaB_Pa	PA2580	64 (MdaB_Ec)	AAG05968.1
WrbA_Pa	PA0949	39 (WrbA_Ec)	AAG04338.1
YcaK1_Pa	PA1225	33 (YcaK_Ec)	AAG04614.1
YcaK2_Pa	PA0853	27 (YcaK_Ec)	AAG04242.1
YdjA_Pa	PA3208	42 (YdjA_Ec)	AAG06596.1
YieF_Pa	PA1204	45 (YieF_Ec)	AAG04593.1
NfsA_Ec	-	100 (NfsA_Ec)	BAA35562.1
NfsB_Ec	-	100 (NfsB_Ec)	AAC73679.1

^aProtein nomenclature as assigned in this study, based on existing nomenclature of closest E. coli orthologue, with the exception of AzoR2 and AzoR3 named as per existing GenBank annotations. Suffix _Pa indicates host of origin was P. aeruginosa PAO1; _Ec indicates E. coli W3110.
^bGene locus ID for P. aeruginosa proteins, as assigned in Pseudomonas Genome Database [14].
^cPercentage amino acid identity shared with closest E. coli orthologue (identified in parentheses).
^dGenBank accession number for protein sequences.

ISSN: 1476-4598