Figure 1

qRT-PCR validation of 18 candidate prolactin modulated transcripts. (A) Representative images of T47D xenografts treated with or without PRL for 48 h and stained with tyrosine phosphorylated Stat5 (pY-Stat5)(red) or DAPI (blue) using immunofluorescence. (B) RNA isolated from T47D xenografts were tested for 18 breast cancer relevant genes using qRT-PCR analysis. Bars represent an average of fold change from 3 independent xenograft RNA samples. (C) Immunoblot of pY-Stat5 and Stat5 in T47D cells grown in vitro and placed in serum starvation media for 24 h before treatment with vehicle or 10 nM PRL for 24 h. (D) RNA isolated from T47D cells grown in vitro were tested for the same 18 breast cancer relevant genes using qRT-PCR analysis. Bars represent an average of fold change from 3 independent experiments.