Figure 3

Jak2 and Jak1 are critical for PRL transcript regulation in T47D breast cancer cells. T47D cells were treated with lentivirus containing NTC, Jak1 shRNA, or Jak2 shRNA overnight. Then, cells were placed in serum starvation media for 24 h and then treated with vehicle or 10 nM PRL for another 24 h before RNA isolation and qRT-PCR analysis of the 12 PRL regulated transcripts.