Figure 4

Enhancement by E2 of PRL-induced proliferation and select PRL modulated transcripts. (A) T47D cells were treated with varying doses of PRL (0, 1, 10, 20, 37, and 100 nM) in the presence of constant E2 (1 nM) for 72 h. Bars represent triplicates of each condition that were counted and averaged. (B) T47D cells were treated with varying doses of E2 (0.001, 0.01, 0.1, 1, 10 nM) in the presence of constant PRL (20 nM) for 72 h. Bars represent triplicates of each condition that were counted and averaged. (C) T47D cells were treated with vehicle, 20 nM PRL, 1 nM E2, or 20 nM PRL + 1 nM E2 for 2 weeks. Images from four independent wells were analyzed for colony size through ImageJ. (D) Representative images of T47D cells grown on soft agar and treated with vehicle, PRL, E2, or PRL + E2 for 2 weeks. (E) T47D cells were treated with PRL, E2, or PRL + E2 for 24 h. RNA was isolated and qRT-PCR analysis was performed on the 12 PRL modulated transcripts.