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Figure 3 | Molecular Cancer

Figure 3

From: Differential G protein subunit expression by prostate cancer cells and their interaction with CXCR5

Figure 3

Validation of G αq/11 , G αi2 , and G α13 protein association with CXCR5. Cell lines were treated with or without CXCL13 and lysed. (A) Gαq/11 and (B) Gαi2 were immunoprecipitated (IP) from total cell lysates. The IP cell lysates were resolved by SDS-PAGE and CXCR5 expression was examined by immunoblot. (C) Identification of CXCR4 and CXCR5 coupled to Gα13 following CXCL13 stimulation. Cell lines were treated with or without CXCL13 and lysed. Antibody against Gα13 was used to immunoprecipitate (IP) it from total cell lysates. The IP cell lysates were resolved by SDS PAGE and immunoblotted for CXCR5 followed by CXCR4, after stripping. In all the experiments, GAPDH served as loading control. All experiments were repeated three times with unvarying results.

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