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Figure 3 | Molecular Cancer

Figure 3

From: Hedgehog signaling regulates hypoxia induced epithelial to mesenchymal transition and invasion in pancreatic cancer cells via a ligand-independent manner

Figure 3

Characteristics of EMT and invasion under hypoxia condition in pancreatic cancer cells. (A) Western blot analysis of EMT related molecules E-cadherin, vimentin, and Snail of PANC-1, BxPC-3 cells under normoxic or hypoxic conditions for 48 h. (B) Matrigel invasion assay. PANC-1 and BxPC-3 cells were seeded into a matrigel-coated invasion chamber under normoxic or hypoxic conditions for 48 h. Left, Representative staining. ×100 magnification. Right, quantification of invasion. The number of migrated cells was quantified by counting the cells from 10 random fields. The data are representative of 3 independent experiments. *P < 0.05. Column: mean (n = 10); bar: SD; *P < 0.05 compared to normal controls.

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