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Figure 4 | Molecular Cancer

Figure 4

From: Hedgehog signaling regulates hypoxia induced epithelial to mesenchymal transition and invasion in pancreatic cancer cells via a ligand-independent manner

Figure 4

HIF-1α interference reverses hypoxia induced Hh signaling activation, EMT process and invasion. PANC-1 and BxPC-3 cells were incubated under hypoxic conditions. Normal control groups were also under hypoxic conditions. (A) Western blot detection of HIF-1α in siRNA-transfected PANC-1 and BxPC-3 cells. (B) qRT-PCR analysis of HIF-1α mRNA level after both cells transfected with siRNA. (C) The effects of HIF-1α siRNA on the expression of SMO and GLI1. SMO and GLI1 expression levels following transfected with siRNA for 48 h were estimated by Western blot. (D) The expression of SHH, PTCH1, SMO and GLI1 mRNA level were evaluated by qRT-PCR following transfection with siRNA for 48 h. (E) The effects of HIF-1α siRNA on EMT process. E-cadherin, vimentin, N-cadherin and Snail protein levels of PANC-1, BxPC-3 cells transfected with siRNA for 48 h were analysed by Western blot. (F) The effect on cell invasion in response to HIF-1α knockdown. After transfection with siRNA for 48 h, PANC-1 and BxPC-3 cells were incubated under hypoxic conditions for 48 h. Control groups were under normoxic condiions. The number of cells was counted under a light microscope.

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