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Figure 5 | Molecular Cancer

Figure 5

From: Hedgehog signaling regulates hypoxia induced epithelial to mesenchymal transition and invasion in pancreatic cancer cells via a ligand-independent manner

Figure 5

The effects of cyclopamine on hypoxia induced pancreatic cancer cell EMT and invasion. PANC-1 and BxPC-3 cells were treated with or without cyclopamine under hypoxia or normal conditions. (A) The effects of cyclopamine on the expression of SMO, GLI1 and EMT-related molecules E-cadherin, vimentin and Snial were estimated by Western blot. (B) The effect on cell invasion in response to inhibition of SMO by cyclopamine. After treated with cyclopamine for 48 h, the cells were seeded into a matrigel-coated invasion chamber under hypoxia or normal conditions for 48 h. The number of migrated cells was quantified by counting the number of cells from 10 random fields at ×100 magnification.

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