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Figure 6 | Molecular Cancer

Figure 6

From: Hedgehog signaling regulates hypoxia induced epithelial to mesenchymal transition and invasion in pancreatic cancer cells via a ligand-independent manner

Figure 6

GLI1 interference abolishes hypoxia induced pancreatic cancer EMT and invasion. After transfection with siRNA for 48 h, PANC-1 and BxPC-3 cells were incubated under normoxic or hypoxic conditions for 48 h. (A) Western blot detection of GLI1 in siRNA-transfected PANC-1 and BxPC-3 cells. (B) qRT-PCR analysis of GLI1 mRNA level after both cells transfected with siRNA. (C) The effects of GLI1 siRNA on SMO and EMT process. SMO, E-cadherin, vimentin and Snail protein levels of PANC-1, BxPC-3 cells transfected with siRNA were analysed by Western blot. (D) The effect on cell invasion in response to GLI1 knockdown. After transfection with siRNA for 48 h, the cells were seeded into a matrigel-coated invasion chamber under normoxic or hypoxic conditions for 48 h. The number of cells was counted under a light microscope.

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Molecular Cancer

ISSN: 1476-4598