Figure 1
From: Role of SIRT3 in the regulation of redox balance during oral carcinogenesis
Variable levels of SIRT3 expression and its activity are noted in normal cells (HOK) and OSCCs. (A) RT-PCR and western blotting reveal the expression levels of SIRT3 in HOK and OSCC cell lines. (B) Specific activities of SIRT3 in HOK and OSCC cell lines were determined by enzyme assay. Each data point represents the mean ± SD from at least 3 independent experiments (mean ± SD). (C) Mitochondrial extracts from HOK, OECM1, and HSC3 cells were immunoprecipitated by using a SIRT3 antibody and analyzed by western blot using antibodies against SOD2, IDH2, and acetylated-lysine (Ac-K).